Algorithmic Tools for Spectral Image Annotation and Registration

Annotating microspectroscopic images by overlaying them with stained microscopic images is an essential task required in many applications of vibrational spectroscopic imaging. This talk introduces two novel tools applicable in this context. First, an image registration approach is presented that allows to locate (register) a spectral image within a larger H+E stained image, which is an essential prerequisite to annotate the spectral image. The second part introduces the interactive Lasagne annotation tool that allows to explore spectral images by highlighting regions sharing high spectral similarity using distance geometry.