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Rabis/etal/2011a: Detection of infectious agents in the airways by ion mobility spectrometry of exhaled breath

Bibtype Article
Bibkey Rabis/etal/2011a
Author Rabis, T. and Sommerwerck, U. and Anhenn, O. and Darwiche, K. and Freitag, L. and Teschler, H. and Bödeker, B. and Maddula, S. and Baumbach, J. I.
Title Detection of infectious agents in the airways by ion mobility spectrometry of exhaled breath
Journal International Journal for Ion Mobility Spectrometry
Abstract Diseases of the lung, e. g. chronic obstructive pulmonary disease (COPD), interstitial lung diseases, bronchi-ectasis or cystic fibrosis, often lead to recurrent severe respiratory infections that cause exacerbations of the underlying disease. These acute or chronic inflammatory processes can result in a progressive destruction of the lung and in an ongoing decline in lung function. Therefore longer inpatient stays for intravenous antibiotic treatment are necessary and the quality of life in these patients is severely limited. A rapid detection of infectious agents in human lungs is often crucial, because the
choice of the appropriate therapeutic regime depends at first on
the identification of the infecting species. Standard methods for
detection and identification are either time consuming, of low
sensitivity or expensive. It is known that bacteria, and also
mitosporic fungi, produce volatile organic compounds (VOCs) that can be detected in exhaled breath by ion mobility spectrometry (IMS), were a distinct detection of a specific VOC is related to a “peak”. We investigated, whether the detection and haracterisation of VOCs by Multi-capillary column coupled to IMS in exhaled breath of patients whose airways are either infected or colonized by Pseudomonas aeruginosa compared to healthy non-smoker controls is
capable of identifying those infectious agents. To realize a non invasive identification of pathogens, the exhaled breath of 53 persons (24 patients suffering chronic or infectious on Pseudomonas and 29 healthy controls) was investigated using an ion mobility spectrometer type BioScout. In total 224 different signals were found. Actually, 21 different signals are able to differentiate the two groups, Control and Pseudomonas, with rank sum values less than 0.2. For all 224 signals Box-and-Wisker plots were realized. The peaks with the lowest rank sum values F (0,107) and PS0 (0,112) show rather good separation of both groups. Our preliminary results demonstrate that distinct patterns of a small number of IMS-peaks are sufficient for the identification of these infectious agents. Therefore MCC-IMS seems to be a promising method
for the non-invasive identification of patients which are colonized or infected with bacteria such as Pseudomonas aeruginosa.
Note DOI 10.1007/s12127-011-0077-6
Year 2011
Projekt SFB876-B1
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